mouse tnf α elisa kit  (R&D Systems)

Journal: Molecular Medicine Reports

Article Title: Veronicastrum sibiricum (L.) Pennell extract alleviates inflammation-induced muscle atrophy through NLRP3 inflammasome regulation and mitochondrial function restoration

doi: 10.3892/mmr.2026.13857

Figure Lengend Snippet: VSE reduces the secretion of proinflammatory cytokines. C2C12 myotubes were pretreated with VSE at 0 (LPS only), 1.25, 2.5 and 5 µg/ml for 3 h and then co-treated with 500 ng/ml LPS for 24 h. Controls received no treatments. (A) mRNA expression levels of the proinflammatory cytokines TNF-α and IL-1β were analyzed using reverse transcription-quantitative PCR. (B) Protein expression levels of the proinflammatory cytokines TNF-α and IL-1β in the supernatant of C2C12 myotube cultures were analyzed using ELISA. All data are presented as the mean ± SD of triplicate results and statistical analysis was carried out using one-way ANOVA with Tukey's post hoc test. #### P<0.0001 vs. control; ***P<0.001, ****P<0.0001 vs. LPS treatment. A450, absorbance at 450 nm; LPS, lipopolysaccharide; VSE, Veronicastrum sibiricum seed extract.

Article Snippet: Supernatants were acquired, and secretion of the proinflammatory cytokines TNF-α (cat. no. MTA00B; R&D systems, Inc.) and IL-1β was analyzed using ELISA kits (cat. no. DY401; R&D Systems, Inc.) according to the manufacturer's guidelines.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control

Journal: Molecular Medicine Reports

Article Title: Veronicastrum sibiricum (L.) Pennell extract alleviates inflammation-induced muscle atrophy through NLRP3 inflammasome regulation and mitochondrial function restoration

doi: 10.3892/mmr.2026.13857

Figure Lengend Snippet: VSE exhibits protective effects against pyroptosis. (A) Mouse serum was obtained 24 h after intraperitoneal LPS administration, diluted to 1:50, and the protein expression levels of proinflammatory cytokines TNF-α and IL-1β were analyzed using ELISAs. (B) GAS muscle tissue was obtained, the mRNA was extracted and the mRNA expression levels of muscle atrophy-related genes MuRF1 and atrogin-1 were analyzed using reverse transcription-quantitative PCR. (C) GAS muscle tissue was obtained, proteins were extracted utilizing PRO-PREP™, and the protein expression levels of muscle atrophy-related and NLRP3 pathway-related proteins were analyzed using western blotting (n=6 per group). All data are presented as the mean ± SD of triplicate results and statistical analysis was carried out using one-way ANOVA followed by Tukey's post hoc test in GraphPad Prism 10. ## P<0.01, ### P<0.001, #### P<0.0001 vs. control; *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001 vs. LPS treatment. LPS, lipopolysaccharide; VSE, Veronicastrum sibiricum seed extract; GAS, gastrocnemius; MuRF1, muscle-specific RING finger protein 1; MaFbx, muscle atrophy F-box protein; MyHC, myosin heavy chain; NLRP3, NLR family pyrin domain containing 3; GSDMD, gasdermin-D; CON, control; A450, absorbance at 450 nm.

Article Snippet: Supernatants were acquired, and secretion of the proinflammatory cytokines TNF-α (cat. no. MTA00B; R&D systems, Inc.) and IL-1β was analyzed using ELISA kits (cat. no. DY401; R&D Systems, Inc.) according to the manufacturer's guidelines.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Control

Journal: Poultry Science

Article Title: Epidemiological investigation and antimicrobial resistance profiling of Salmonella in broiler chickens with assessment of Lactococcus lactis probiotic therapy

doi: 10.1016/j.psj.2025.105967

Figure Lengend Snippet: Effect of Lactococcus lactis IL403 on TNF-α release by murine macrophages. RAW 264.7 cells were stimulated with lipopolysaccharide (LPS) alone as a positive control. Co-treatment with L. lactis IL403 significantly reduced LPS-induced TNF-α secretion. Data represent mean ± SD from three independent experiments. Statistical significance compared to LPS-only control is indicated ( p < 0.05).

Article Snippet: TNF-α concentrations were detected with Mouse TNF-α Quantikine ELISA Kits (R&D Systems, USA) according to the manufacturer’s protocols.

Techniques: Positive Control, Control

Journal: PLOS One

Article Title: Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19

doi: 10.1371/journal.pone.0348065

Figure Lengend Snippet: Male and female hACE2/Poldip2 mice received a nasal instillation of PBS or SARS-CoV-2. BAL fluid and lungs were collected after 7 days and processed as in Figure 6 to measure the expression of cytokine mRNAs in lung tissue (A) and proteins in BAL (B). Bars represent means ± SEM of data from n = 5-19 animals. Basal protein levels in +/ + PBS were 1.7 ± 0.1 pg/ml (TNFα), 5.0 ± 0.3 pg/ml (IL-1β), 11.8 ± 0.8 pg/ml (IL-6), 4.2 ± 0.5 pg/ml (IFN-γ). Data were analyzed using 2-way ANOVA: ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: Specific mouse sandwich enzyme-linked immunosorbent assays (ELISA) were carried out using kits from R&D Systems: TNFα (MHSTA50), IL-1B/IL-1F2 (MHSLB00), IL-6 (M6000B-1), MCP1/CCL2 (MJE00B), CXCL1/KC (MKC00B-1), IFN-γ (MIF00–1).

Techniques: Expressing